犬乳腺肿瘤中MUC1的表达及临床意义

doi:10.11843/j.issn.0366-6964.2018.07.021

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (7): 1511-1516.doi: 10.11843/j.issn.0366-6964.2018.07.021

犬乳腺肿瘤中MUC1的表达及临床意义

刁洪秀, 李艺, 林德贵, 张迪*

中国农业大学动物医学院, 北京 100193

收稿日期:2018-01-08 出版日期:2018-07-23 发布日期:2018-07-23
通讯作者: 张迪,副教授,E-mail:dzhangdvm@cau.edu.cn
作者简介:刁洪秀(1989-),女,吉林长春人,博士生,主要从事小动物外科学和肿瘤学研究,E-mail:diaohongxiu@yeah.net
基金资助:
国家自然科学基金青年基金项目（31402260）

Expression of MUC1 and Its Clinical Value in Canine Mammary Tumors

DIAO Hong-xiu, LI Yi, LIN De-gui, ZHANG Di*

College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received:2018-01-08 Online:2018-07-23 Published:2018-07-23

摘要/Abstract

摘要：

为了检测MUC1在乳腺肿瘤的侵袭、转移及病理分级中的作用，本研究通过real-time PCR检测了47例犬乳腺肿瘤组织（20例良性，27例恶性）和3例健康犬乳腺组织中MUC1基因的转录情况，通过IHC检测MUC1在犬乳腺组织中的表达情况，并分析二者与肿瘤恶性程度分级的关系。结果发现：MUC1基因和MUC1在犬乳腺肿瘤组织中高表达，且在不同的恶性分级中差异显著（P<0.05）。研究显示MUC1基因和MUC1可作为犬乳腺肿瘤诊断和恶性程度评估的重要指标之一。

Abstract:

This study was conducted to assess the influences of MUC1 in cancer invasion, metastasis, classification and grade in mammary tumors. This work was designed to investigate the expression of MUC1 mRNA and MUC1 in canine mammary tissues. Forty-seven canine mammary gland tumors(20 benign and 27 malignant mammary tumors) and mammary gland tissues from 3 healthy dogs were investigated for MUC1 mRNA and MUC1 by Real-Time PCR and immunohistochemistry(IHC), respectively. The results showed that the expression levels of MUC1 gene and MUC1 in canine mammary tumors were significant higher than that in healthy mammary gland(P<0.05), and a significant correlation was observed between the expression levels of MUC1 gene and MUC1 and malignance grades. In conclusion, the expression levels of MUC1 gene and MUC1 may become new and valuable biomarkers for diagnosis and malignance grade in canine mammary tumors.

中图分类号:

S857.26

刁洪秀, 李艺, 林德贵, 张迪. 犬乳腺肿瘤中MUC1的表达及临床意义[J]. 畜牧兽医学报, 2018, 49(7): 1511-1516.

DIAO Hong-xiu, LI Yi, LIN De-gui, ZHANG Di. Expression of MUC1 and Its Clinical Value in Canine Mammary Tumors[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1511-1516.

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犬乳腺肿瘤中MUC1的表达及临床意义

Expression of MUC1 and Its Clinical Value in Canine Mammary Tumors

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